Both nanotechnology and immune-oncology fields have motivated Florindo’s research, which has been focused on the rational development and characterization of disruptive chemically defined functionalized nanobiomaterials as novel immunotherapies for cancer and infectious diseases. We are interested in identifying process parameters critical for targeted nanoparticle quality attributes, identified as optimal for targeting specific immune cell subtypes in pharmacological active sites. To this end, Florindo’s team follows an iterative approach to evaluate the impact of the physicochemical properties of these delivery systems on their interaction with targeted cells. These studies dissect the transport mechanisms and effect on dendritic cell (DC) maturation and T/B cell activation of these multi-component immune-modulators, as well as their biodistribution profiles and pharmacokinetics, in vivo efficacy and effect on immune and stromal cell functions.